The Pathways of Plant Water Movement

Experiment 6- The Pathways of Plant Water Movement Aim To inquire the movement of materials in xylem Materials ?Fresh celery radical with leaves ?Eosin root (1%) ?A plastic container with tap weewee ?A sharp scalpel ? tidy sum lens ?A light microscope ?Two glass slides ?A palm slip Risk Assessment and Safety Precautions Risk AssessmentSafety Precautions Eosin solution- burn down cause stainsAlways use gloves when handling Scalpel is dangerous and can cause cutsAlways underwrite carefully, with blade facing down. Be especially careful when walking with a scalpel.Glassware can break easilyBe careful with all glassware. If cut, wash with water and stress advice to avoid infection. Method 1. Obtain a fresh celery stalk and used a sharp scalpel to make a 45 period angle cut under water, to prevent the air bubbles from blocking the xylem. straightway placed the celery stalk in eosin solution and remaining it overnight. 2. Removed celery stalk from the eosin solution and used a sca lpel to cut a very thin, transverse section of the celery stop. 3. placed the thin sample on a clean slide and cover it with a cover slip.Observed the sample under amply magnification victimization a light microscope. 4. Drew the observation under a high power. 5. Located one vascular bundle and peeled it longitudinally in the varnished region. Made a wet mount of the longitudinal create from raw material and observed it under a microscope. Results Transverse (Low)Transverse (High) X40X400 Longitudinal (High) X400 Questions/ parole 1)What general stem structures were stained? What tissues in these structures were most heavily stained? The general stem structures stained were the cell walls of the xylem. The base of the celery stem was most heavily stained. )What statement can you make regarding the transpirational pathway of water in a plant? Even root distribution is grave for both water and nutrient uptake, because of the movement of water and nutrients in the dry land and in the plant xylem,, even root distribution is important. The The xylem pathway is a continuous column of water, which is not broken by bubbles, and contained in a cellulose pipe which the walls are porous in places. 3)What was the heading of this exercise? The purpose of this exercise was to observe and investigate the movement of materials (in this case, water) in xylem.